6z0w

Crystal structure of the cytoplasmic domain of FlhB from Shewanella putrefaciens

spinqualitylabelsall models 6z0w, resolution 2.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6z0w is a 4 chain structure with sequence from Shewanella putrefaciens CN-32. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.1Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A4Y8K1_SHEPC Required for formation of the rod structure in the basal body of the flagellar apparatus. Together with FliI and FliH, may constitute the export apparatus of flagellin.[ARBA:ARBA00025078][RuleBase:RU364091] Publication Abstract from PubMed Flagella are bacterial organelles of locomotion. Their biogenesis is highly coordinated in time and space and relies on a specialized flagellar type III secretion system (fT3SS) required for the assembly of the extracellular hook, rod, and filament parts of this complex motor device. The fT3SS protein FlhB switches secretion substrate specificity once the growing hook reaches its determined length. Here we present the crystal structure of the cytoplasmic domain of the transmembrane protein FlhB. The structure visualizes a so-far unseen proline-rich region (PRR) at the very C-terminus of the protein. Strains lacking the PRR show a decrease in flagellation as determined by hook- and filament staining, indicating a role of the PRR during assembly of the hook and filament structures. Phylogenetic analysis shows that the PRR is a primary feature of FlhB proteins of flagellated beta- and gamma-proteobacteria. Taken together, our study adds another layer of complexity and organismic diversity to the process of flagella biogenesis. A Proline-Rich Element in the Type III Secretion Protein FlhB Contributes to Flagellar Biogenesis in the Beta- and Gamma-Proteobacteria.,Hook JC, Blagotinsek V, Pane-Farre J, Mrusek D, Altegoer F, Dornes A, Schwan M, Schier L, Thormann KM, Bange G Front Microbiol. 2020 Dec 15;11:564161. doi: 10.3389/fmicb.2020.564161., eCollection 2020. PMID:33384667 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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