6z2q

Crystal structure of wild type OgpA from Akkermansia muciniphila in complex with an O-glycopeptide (GalGalNAc-TS) product

spinqualitylabelsall models 6z2q, resolution 2.35Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6z2q is a 2 chain structure with sequence from Akkermansia muciniphila ATCC BAA-835 and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.347Å Ligands: A2G, ACY, EDO, GAL, PRD_900084, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function B2UR60_AKKM8 Publication Abstract from PubMed Akkermansia muciniphila is a mucin-degrading bacterium commonly found in the human gut that promotes a beneficial effect on health, likely based on the regulation of mucus thickness and gut barrier integrity, but also on the modulation of the immune system. In this work, we focus in OgpA from A. muciniphila, an O-glycopeptidase that exclusively hydrolyzes the peptide bond N-terminal to serine or threonine residues substituted with an O-glycan. We determine the high-resolution X-ray crystal structures of the unliganded form of OgpA, the complex with the glycodrosocin O-glycopeptide substrate and its product, providing a comprehensive set of snapshots of the enzyme along the catalytic cycle. In combination with O-glycopeptide chemistry, enzyme kinetics, and computational methods we unveil the molecular mechanism of O-glycan recognition and specificity for OgpA. The data also contribute to understanding how A. muciniphila processes mucins in the gut, as well as analysis of post-translational O-glycosylation events in proteins. Structural basis of mammalian mucin processing by the human gut O-glycopeptidase OgpA from Akkermansia muciniphila.,Trastoy B, Naegeli A, Anso I, Sjogren J, Guerin ME Nat Commun. 2020 Sep 24;11(1):4844. doi: 10.1038/s41467-020-18696-y. PMID:32973204 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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