6zfc

Fucose-binding lectin from Burkholderia ambifaria (BamBL) in complex with a fucosyl derivative

spinqualitylabelsall models 6zfc, resolution 1.65Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6zfc is a 6 chain structure with sequence from Burkholderia ambifaria AMMD. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.65Å Ligands: QJB Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q0B4G1_BURCM Publication Abstract from PubMed Two orthogonal, metal free click reactions, enabled to glycosylate ubiquitin and its mutant A28C forming two protein scaffolds with high affinity for BambL, a lectin from the human pathogen Burkholderia ambifaria. A new fucoside analogue, with high affinity with BambL, firstly synthetized and co-crystallized with the protein target, provided the insights for sugar determinants grafting onto ubiquitin. Three ubiquitin-based glycosides were thus assembled. Fuc-Ub, presented several copies of the fucoside analogue, with proper geometry for multivalent effect; Rha-A28C, displayed one thio-rhamnose, known for its ability to tuning the immunological response; finally, Fuc-Rha-A28C, included both multiple fucoside analogs and the rhamnose residue. Fuc-Ub and Fuc-Rha-A28C ligands proved high affinity for BambL and unprecedented immune modulatory properties towards macrophages activation. Fucosylated ubiquitin and orthogonally glycosylated mutant A28C: conceptually new ligands for Burkholderia ambifaria lectin (BambL).,Kuhaudomlarp S, Cerofolini L, Santarsia S, Gillon E, Fallarini S, Lombardi G, Denis M, Giuntini S, Valori C, Fragai M, Imberty A, Dondoni A, Nativi C Chem Sci. 2020 Oct 21;11(47):12662-12670. doi: 10.1039/d0sc03741a. PMID:34094460 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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