6zg3 | pdb_00006zg3

the structure of ECF PanT transporter in a complex with a nanobody

spinqualitylabelsall models 6zg3, resolution 2.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6zg3 is a 10 chain structure with sequence from Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 = JCM 1002 and Lama glama. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.8Å Ligands: CIT, LMT, PEG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function ECFA1_LACDA ATP-binding (A) component of a common energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates. Publication Abstract from PubMed Energy-coupling factor (ECF) transporters mediate import of micronutrients in prokaryotes. They consist of an integral membrane S-component (that binds substrate) and ECF module (that powers transport by ATP hydrolysis). It has been proposed that different S-components compete for docking onto the same ECF module, but a minimal liposome-reconstituted system, required to substantiate this idea, is lacking. Here, we co-reconstituted ECF transporters for folate (ECF-FolT2) and pantothenate (ECF-PanT) into proteoliposomes, and assayed for crosstalk during active transport. The kinetics of transport showed that exchange of S-components is part of the transport mechanism. Competition experiments suggest much slower substrate association with FolT2 than with PanT. Comparison of a crystal structure of ECF-PanT with previously determined structures of ECF-FolT2 revealed larger conformational changes upon binding of folate than pantothenate, which could explain the kinetic differences. Our work shows that a minimal in vitro system with two reconstituted transporters recapitulates intricate kinetics behaviour observed in vivo. In vitro reconstitution of dynamically interacting integral membrane subunits of energy-coupling factor transporters.,Setyawati I, Stanek WK, Majsnerowska M, Swier LJYM, Pardon E, Steyaert J, Guskov A, Slotboom DJ Elife. 2020 Dec 22;9:e64389. doi: 10.7554/eLife.64389. PMID:33350937 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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