6zn8

Crystal structure of the H. influenzae VapXD toxin-antitoxin complex

spinqualitylabelsall models 6zn8, resolution 3.21Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6zn8 is a 6 chain structure with sequence from Haemophilus influenzae 86-028NP. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.211Å Ligands: MSE Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q4QN95_HAEI8 Cleaves ssRNA, mostly between U:A.[PIRNR:PIRNR002882] Publication Abstract from PubMed Bacterial type II toxin-antitoxin (TA) modules encode a toxic protein that downregulates metabolism and a specific antitoxin that binds and inhibits the toxin during normal growth. In non-typeable Haemophilus influenzae, a common cause of infections in humans, the vapXD locus was found to constitute a functional TA module and contribute to pathogenicity; however, the mode of action of VapD and the mechanism of inhibition by the VapX antitoxin remain unknown. Here, we report the structure of the intact H. influenzae VapXD complex, revealing an unusual 2:1 TA molecular stoichiometry where a Cas2-like homodimer of VapD binds a single VapX antitoxin. VapX consists of an oligonucleotide/oligosaccharide-binding domain that docks into an asymmetrical cavity on the toxin dimer. Structures of isolated VapD further reveal how a symmetrical toxin homodimer adapts to interacting with an asymmetrical antitoxin and suggest how a primordial TA system evolved to become part of CRISPR-Cas immunity systems. Structural Basis for Toxin Inhibition in the VapXD Toxin-Antitoxin System.,Bertelsen MB, Senissar M, Nielsen MH, Bisiak F, Cunha MV, Molinaro AL, Daines DA, Brodersen DE Structure. 2020 Oct 16. pii: S0969-2126(20)30373-7. doi:, 10.1016/j.str.2020.10.002. PMID:33096014 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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