6znp

Crystal Structure of DUF1998 helicase MrfA bound to DNA

spinqualitylabelsall models 6znp, resolution 3.16Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6znp is a 4 chain structure with sequence from Bacillus subtilis subsp. subtilis str. 168 and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.16Å Ligands: CIT, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function YPRA_BACSU Publication Abstract from PubMed Mitomycin repair factor A represents a family of DNA helicases that harbor a domain of unknown function (DUF1998) and support repair of mitomycin C-induced DNA damage by presently unknown molecular mechanisms. We determined crystal structures of Bacillus subtilis Mitomycin repair factor A alone and in complex with an ATP analog and/or DNA and conducted structure-informed functional analyses. Our results reveal a unique set of auxiliary domains appended to a dual-RecA domain core. Upon DNA binding, a Zn2+-binding domain, encompassing the domain of unknown function, acts like a drum that rolls out a canopy of helicase-associated domains, entrapping the substrate and tautening an inter-domain linker across the loading strand. Quantification of DNA binding, stimulated ATPase and helicase activities in the wild type and mutant enzyme variants in conjunction with the mode of coordination of the ATP analog suggest that Mitomycin repair factor A employs similar ATPase-driven conformational changes to translocate on DNA, with the linker ratcheting through the nucleotides like a 'skipping rope'. The electrostatic surface topology outlines a likely path for the displaced DNA strand. Our results reveal unique molecular mechanisms in a widespread family of DNA repair helicases linked to bacterial antibiotics resistance. A skipping rope translocation mechanism in a widespread family of DNA repair helicases.,Roske JJ, Liu S, Loll B, Neu U, Wahl MC Nucleic Acids Res. 2020 Dec 9. pii: 6029183. doi: 10.1093/nar/gkaa1174. PMID:33300032 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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