6zzc

MB_CRS6-1 bound to CrSAS-6_6HR

spinqualitylabelsall models 6zzc, resolution 2.93Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6zzc is a 6 chain structure with sequence from Chlamydomonas reinhardtii and Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.93Å Ligands: 12P Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A9CQL4_CHLRE Publication Abstract from PubMed Centrioles are evolutionarily conserved multi-protein organelles essential for forming cilia and centrosomes. Centriole biogenesis begins with self-assembly of SAS-6 proteins into 9-fold symmetrical ring polymers, which then stack into a cartwheel that scaffolds organelle formation. The importance of this architecture has been difficult to decipher notably because of the lack of precise tools to modulate the underlying assembly reaction. Here, we developed monobodies against Chlamydomonas reinhardtii SAS-6, characterizing three in detail with X-ray crystallography, atomic force microscopy and cryo-electron microscopy. This revealed distinct monobody-target interaction modes, as well as specific consequences on ring assembly and stacking. Of particular interest, monobody MB(CRS6)-15 induces a conformational change in CrSAS-6, resulting in the formation of a helix instead of a ring. Furthermore, we show that this alteration impairs centriole biogenesis in human cells. Overall, our findings identify monobodies as powerful molecular levers to alter the architecture of multi-protein complexes and tune centriole assembly. Tuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly.,Hatzopoulos GN, Kukenshoner T, Banterle N, Favez T, Fluckiger I, Hamel V, Andany S, Fantner GE, Hantschel O, Gonczy P Nat Commun. 2021 Jun 21;12(1):3805. doi: 10.1038/s41467-021-23897-0. PMID:34155202 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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