7a00
Crystal structure of Shank1 PDZ in complex with L6F mutant of the C-terminal hexapeptide from GKAP
Structural highlights
FunctionSHAN1_HUMAN Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction. Publication Abstract from PubMedProtein-protein interactions (PPIs) are central to biological mechanisms, and can serve as compelling targets for drug discovery. Yet, the discovery of small molecule inhibitors of PPIs remains challenging given the large and typically shallow topography of the interacting protein surfaces. Here, we describe a general approach to the discovery of orthosteric PPI inhibitors that mimic specific secondary protein structures. Initially, hot residues at protein-protein interfaces are identified in silico or from experimental data, and incorporated into secondary structure-based queries. Virtual libraries of small molecules are then shape-matched against the queries, and promising ligands docked to target proteins. The approach is exemplified experimentally using two unrelated PPIs that are mediated by an alpha-helix (p53/hDM2) and a beta-strand (GKAP/SHANK1-PDZ). In each case, selective PPI inhibitors are discovered with low muM activity as determined by a combination of fluorescence anisotropy and (1)H-(15)N HSQC experiments. In addition, hit expansion yields a series of PPI inhibitors with defined structure-activity relationships. It is envisaged that the generality of the approach will enable discovery of inhibitors of a wide range of unrelated secondary structure-mediated PPIs. Query-guided protein-protein interaction inhibitor discovery.,Celis S, Hobor F, James T, Bartlett GJ, Ibarra AA, Shoemark DK, Hegedus Z, Hetherington K, Woolfson DN, Sessions RB, Edwards TA, Andrews DM, Nelson A, Wilson AJ Chem Sci. 2021 Mar 2;12(13):4753-4762. doi: 10.1039/d1sc00023c. PMID:34163731[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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