7aef

Cryo-EM structure of an extracellular contractile injection system in marine bacterium Algoriphagus machipongonensis, the baseplate complex in extended state applied 3-fold symmetry.

spinqualitylabelsall models 7aef, resolution 2.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7aef is a 48 chain structure with sequence from Algoriphagus machipongonensis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.8Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A3HTB3_9BACT Publication Abstract from PubMed Contractile injection systems (CISs) are phage tail-like nanomachines, mediating bacterial cell-cell interactions as either type VI secretion systems (T6SSs) or extracellular CISs (eCISs). Bioinformatic studies uncovered a phylogenetic group of hundreds of putative CIS gene clusters that are highly diverse and widespread; however, only four systems have been characterized. Here we studied a putative CIS gene cluster in the marine bacterium Algoriphagus machipongonensis. Using an integrative approach, we show that the system is compatible with an eCIS mode of action. Our cryo-electron microscopy structure revealed several features that differ from those seen in other CISs: a 'cap adaptor' located at the distal end, a 'plug' exposed to the tube lumen, and a 'cage' formed by massive extensions of the baseplate. These elements are conserved in other CISs, and our genetic tools identified that they are required for assembly, cargo loading and function. Furthermore, our atomic model highlights specific evolutionary hotspots and will serve as a framework for understanding and re-engineering CISs. Identification and structure of an extracellular contractile injection system from the marine bacterium Algoriphagus machipongonensis.,Xu J, Ericson CF, Lien YW, Rutaganira FUN, Eisenstein F, Feldmuller M, King N, Pilhofer M Nat Microbiol. 2022 Feb 14. pii: 10.1038/s41564-022-01059-2. doi:, 10.1038/s41564-022-01059-2. PMID:35165385 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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