7b5i

Cryo-EM structure of the contractile injection system cap complex from Anabaena PCC7120

spinqualitylabelsall models 7b5i, resolution 2.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7b5i is a 30 chain structure with sequence from Nostoc sp. PCC 7120 = FACHB-418. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.8Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q8YRW5_NOSS1 Publication Abstract from PubMed Contractile injection systems (CISs) mediate cell-cell interactions by phage tail-like structures, using two distinct modes of action: extracellular CISs are released into the medium, while type 6 secretion systems (T6SSs) are attached to the cytoplasmic membrane and function upon cell-cell contact. Here, we characterized a CIS in the multicellular cyanobacterium Anabaena, with features distinct from extracellular CISs and T6SSs. Cryo-electron tomography of focused ion beam-milled cells revealed that CISs were anchored in thylakoid membrane stacks, facing the cell periphery. Single particle cryo-electron microscopy showed that this unique in situ localization was mediated by extensions of tail fibre and baseplate components. On stress, cyanobacteria induced the formation of ghost cells, presenting thylakoid-anchored CISs to the environment. Functional assays suggest that these CISs may mediate ghost cell formation and/or interactions of ghost cells with other organisms. Collectively, these data provide a framework for understanding the evolutionary re-engineering of CISs and potential roles of these CISs in cyanobacterial programmed cell death. Structure of a thylakoid-anchored contractile injection system in multicellular cyanobacteria.,Weiss GL, Eisenstein F, Kieninger AK, Xu J, Minas HA, Gerber M, Feldmuller M, Maldener I, Forchhammer K, Pilhofer M Nat Microbiol. 2022 Mar;7(3):386-396. doi: 10.1038/s41564-021-01055-y. Epub 2022 , Feb 14. PMID:35165386 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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