7bj5
Inulosucrase from Halalkalicoccus jeotgali
Structural highlights
Publication Abstract from PubMedSeveral archaea harbour genes that code for fructosyltransferase (FTF) enzymes. These enzymes have not been characterized yet at structure-function level, but are of extreme interest in view of their potential role in the synthesis of novel compounds for food, nutrition and pharmaceutical applications. In this study, 3D structure of an inulin-type fructan producing enzyme, inulosucrase (InuHj), from the archaeon Halalkalicoccus jeotgali was resolved in its apo form as well as with bound substrate (sucrose) molecule and first transglycosylation product (1-kestose). This is the first crystal structure of an FTF from halophilic archaea. Its overall five-bladed beta-propeller fold is conserved with previously reported FTFs, but also shows some unique features. The InuHj structure is closer to those of Gram-negative bacteria, with exceptions such as residue E266, which is conserved in FTFs of Gram-positive bacteria and has possible role in fructan polymer synthesis in these bacteria as compared to fructooligosaccharide (FOS) production by FTFs of Gram-negative bacteria. Highly negative electrostatic surface potential of InuHj, due to a large amount of acidic residues, likely contributes to its halophilicity. The complex of InuHj with 1-kestose indicates that the residues D287 in the 4B-4C loop, Y330 in 4D-5A and D361 in the unique alpha2 helix may interact with longer FOSs and facilitate the binding of longer FOS chains during synthesis. The outcome of this work will provide targets for future structure-function studies of FTF enzymes, particularly those from archaea. Crystal structure of an inulosucrase from Halalkalicoccus jeotgali B3T-a halophilic archaeal strain.,Ghauri K, Pijning T, Munawar N, Ali H, Ghauri MA, Anwar MA, Wallis R FEBS J. 2021 Mar 30. doi: 10.1111/febs.15843. PMID:33783128[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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