7bmh

Crystal structure of a light-driven proton pump LR (Mac) from Leptosphaeria maculans

spinqualitylabelsall models 7bmh, resolution 2.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7bmh is a 2 chain structure with sequence from Leptosphaeria maculans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.2Å Ligands: LFA, LYR, OLA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q9HGT7_LEPMC Publication Abstract from PubMed Rhodopsins, most of which are proton pumps generating transmembrane electrochemical proton gradients, span all three domains of life, are abundant in the biosphere, and could play a crucial role in the early evolution of life on earth. Whereas archaeal and bacterial proton pumps are among the best structurally characterized proteins, rhodopsins from unicellular eukaryotes have not been well characterized. To fill this gap in the current understanding of the proton pumps and to gain insight into the evolution of rhodopsins using a structure-based approach, we performed a structural and functional analysis of the light-driven proton pump LR (Mac) from the pathogenic fungus Leptosphaeria maculans. The first high-resolution structure of fungi rhodopsin and its functional properties reveal the striking similarity of its membrane part to archaeal but not to bacterial rhodopsins. We show that an unusually long N-terminal region stabilizes the protein through direct interaction with its extracellular loop (ECL2). We compare to our knowledge all available structures and sequences of outward light-driven proton pumps and show that eukaryotic and archaeal proton pumps, most likely, share a common ancestor. Structure-based insights into evolution of rhodopsins.,Zabelskii D, Dmitrieva N, Volkov O, Shevchenko V, Kovalev K, Balandin T, Soloviov D, Astashkin R, Zinovev E, Alekseev A, Round E, Polovinkin V, Chizhov I, Rogachev A, Okhrimenko I, Borshchevskiy V, Chupin V, Buldt G, Yutin N, Bamberg E, Koonin E, Gordeliy V Commun Biol. 2021 Jun 30;4(1):821. doi: 10.1038/s42003-021-02326-4. PMID:34193947 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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