7bt9
Mevo lectin complex with alpha-mannose
Structural highlights
FunctionPublication Abstract from PubMedCrystallographic and solution studies of Mevo lectin and its complexes, the first effort of its kind on an archeal lectin, reveal a structure similar to beta-prism I fold lectins from plant and animal sources, but with a quaternary association involving a ring structure with seven-fold symmetry. Each subunit in the heptamer carries one sugar binding site on the first Greek key motif. The oligomeric interface is primarily made up of a parallel beta-sheet involving a strand of Greek key Iota of one subunit and Greek key IotaIotaIota from a neighbouring subunit. The crystal structures of the complexes of the lectin with mannose, alphaMan(1,2)alphaMan, alphaMan(1,3)alphaMan, a mannotriose and a mannopentose revealed a primary binding site similar to that found in other mannose specific beta-prism I fold lectins. The complex with alphaMan(1,3)alphaMan provides an interesting case in which a few subunits have the reducing end at the primary binding site, while the majority have the non-reducing end at the primary binding site. The structures of complexes involving the trisaccharide and the pentasaccharide exhibit cross-linking among heptameric molecules. The observed arrangements maybe relevant to the multivalency of the lectin. Phylogenetic analysis of amino acid sequences indicates that Mevo lectin is closer to beta-prism I fold animal lectins than with those of plant origin. The results presented here reinforce the conclusion regarding the existence of lectins in all three domains of life. It would also appear that lectins evolved to the present form before the three domains diverged. Structural and related studies on Mevo lectin from Methanococcus voltae A3. The first thorough characterisation of an archeal lectin and its interactions.,Sivaji N, Suguna K, Surolia A, Vijayan M Glycobiology. 2020 Jul 11. pii: 5869325. doi: 10.1093/glycob/cwaa063. PMID:32651948[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
| ||||||||||||||||||||
