7byf

The crystal structure of mouse ORF10-Rae1-Nup98 complex

spinqualitylabelsall models 7byf, resolution 2.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7byf is a 6 chain structure with sequence from Murid gammaherpesvirus 4 and Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.5Å Ligands: HG, NA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RAE1L_MOUSE Plays a role in mitotic bipolar spindle formation. Binds mRNA. May function in nucleocytoplasmic transport and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton.[UniProtKB:P78406] Publication Abstract from PubMed Viruses employ multiple strategies to inhibit host mRNA nuclear export. Distinct to the generally nonselective inhibition mechanisms, ORF10 from gammaherpesviruses inhibits mRNA export in a transcript-selective manner by interacting with Rae1 (RNA export 1) and Nup98 (nucleoporin 98). We now report the structure of ORF10 from MHV-68 (murine gammaherpesvirus 68) bound to the Rae1-Nup98 heterodimer, thereby revealing detailed intermolecular interactions. Structural and functional assays highlight that two highly conserved residues of ORF10, L60 and M413, play critical roles in both complex assembly and mRNA export inhibition. Interestingly, although ORF10 occupies the RNA-binding groove of Rae1-Nup98, the ORF10-Rae1-Nup98 ternary complex still maintains a comparable RNA-binding ability due to the ORF10-RNA direct interaction. Moreover, mutations on the RNA-binding surface of ORF10 disrupt its function of mRNA export inhibition. Our work demonstrates the molecular mechanism of ORF10-mediated selective inhibition and provides insights into the functions of Rae1-Nup98 in regulating host mRNA export. Molecular mechanism underlying selective inhibition of mRNA nuclear export by herpesvirus protein ORF10.,Feng H, Tian H, Wang Y, Zhang Q, Lin N, Liu S, Yu Y, Deng H, Gao P Proc Natl Acad Sci U S A. 2020 Oct 27;117(43):26719-26727. doi: , 10.1073/pnas.2007774117. Epub 2020 Oct 8. PMID:33033226 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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