7c51

Crystal structure of a Simpl-like protein from Campylobacter jejuni (native protein)

spinqualitylabelsall models 7c51, resolution 2.46Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7c51 is a 1 chain structure with sequence from Campylobacter jejuni. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.46Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A3Z9IF87_CAMJU Publication Abstract from PubMed Signaling molecule that interacts with mouse pelle-like kinase (Simpl) is an animal protein that contributes to the regulation of inflammatory responses. Although Simpl-like proteins (SLPs) are mainly found in bacteria, functional and structural studies on bacterial SLPs are limited to BP26, a periplasmic protein from Brucella species. We identified a group of bacterial SLPs, including Campylobacter jejuni SLP (cjSLP) and Shewanella putrefaciens SLP (spSLP), that exhibit significant sequence variation from Simpl and BP26. To address the structural and oligomeric diversities of SLPs, we determined the crystal structure of cjSLP and performed a comparative analysis of SLP structures. cjSLP adopts a boomerang-shaped, two-domain structure, and each domain of cjSLP adopts an alpha-helix-decorated beta-sheet structure as observed in BP26. This observation suggests that the duplicated alpha/beta structure would be the canonical fold of the Simpl family. Despite the fold similarity, cjSLP exhibits a more open interdomain organization than BP26 and displays unique local structural features that are not observed in BP26. Furthermore, cjSLP and its ortholog spSLP are monomeric in solution in contrast to the hexadecameric assembly of BP26. Therefore, we conclude that cjSLP represents a unique bacterial SLP group that is distinct from BP26 in both structures and oligomeric states. Structural analysis of a Simpl-like protein from Campylobacter jejuni.,Oh HB, Yoon SI Biochem Biophys Res Commun. 2020 Aug 20;529(2):270-276. doi:, 10.1016/j.bbrc.2020.05.211. Epub 2020 Jun 23. PMID:32703422 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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