| Structural highlights
Function
ATR1_HYAAE Secreted effector that acts as an elicitor of hypersensitive response (HR) specifically on plants carrying both defense protein RPP1 from several ecotypes including RPP1-NdA, RPP1-WsB, RPP1-EstA and RPP1-ZdrA.[1] [2] [3] [4] [5] [6]
Publication Abstract from PubMed
Direct or indirect recognition of pathogen-derived effectors by plant nucleotide-binding leucine-rich repeat (LRR) receptors (NLRs) initiates innate immune responses. The Hyaloperonospora arabidopsidis effector ATR1 activates the N-terminal Toll-interleukin-1 receptor (TIR) domain of Arabidopsis NLR RPP1. We report a cryo-electron microscopy structure of RPP1 bound by ATR1. The structure reveals a C-terminal jelly roll/Ig-like domain (C-JID) for specific ATR1 recognition. Biochemical and functional analyses show that ATR1 binds to the C-JID and the LRRs to induce an RPP1 tetrameric assembly required for nicotinamide adenine dinucleotide hydrolase (NADase) activity. RPP1 tetramerization creates two potential active sites, each formed by an asymmetric TIR homodimer. Our data define the mechanism of direct effector recognition by a plant NLR leading to formation of a signaling-active holoenzyme.
Direct pathogen-induced assembly of an NLR immune receptor complex to form a holoenzyme.,Ma S, Lapin D, Liu L, Sun Y, Song W, Zhang X, Logemann E, Yu D, Wang J, Jirschitzka J, Han Z, Schulze-Lefert P, Parker JE, Chai J Science. 2020 Dec 4;370(6521). pii: 370/6521/eabe3069. doi:, 10.1126/science.abe3069. PMID:33273071[7]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Rehmany AP, Gordon A, Rose LE, Allen RL, Armstrong MR, Whisson SC, Kamoun S, Tyler BM, Birch PR, Beynon JL. Differential recognition of highly divergent downy mildew avirulence gene alleles by RPP1 resistance genes from two Arabidopsis lines. Plant Cell. 2005 Jun;17(6):1839-50. PMID:15894715 doi:10.1105/tpc.105.031807
- ↑ Krasileva KV, Zheng C, Leonelli L, Goritschnig S, Dahlbeck D, Staskawicz BJ. Global analysis of Arabidopsis/downy mildew interactions reveals prevalence of incomplete resistance and rapid evolution of pathogen recognition. PLoS One. 2011;6(12):e28765. doi: 10.1371/journal.pone.0028765. Epub 2011 Dec 14. PMID:22194907 doi:https://dx.doi.org/10.1371/journal.pone.0028765
- ↑ Steinbrenner AD, Goritschnig S, Staskawicz BJ. Recognition and activation domains contribute to allele-specific responses of an Arabidopsis NLR receptor to an oomycete effector protein. PLoS Pathog. 2015 Feb 11;11(2):e1004665. PMID:25671309 doi:10.1371/journal.ppat.1004665
- ↑ Goritschnig S, Steinbrenner AD, Grunwald DJ, Staskawicz BJ. Structurally distinct Arabidopsis thaliana NLR immune receptors recognize tandem WY domains of an oomycete effector. New Phytol. 2016 May;210(3):984-96. PMID:26725254 doi:10.1111/nph.13823
- ↑ Schreiber KJ, Bentham A, Williams SJ, Kobe B, Staskawicz BJ. Multiple Domain Associations within the Arabidopsis Immune Receptor RPP1 Regulate the Activation of Programmed Cell Death. PLoS Pathog. 2016 Jul 18;12(7):e1005769. PMID:27427964 doi:10.1371/journal.ppat.1005769
- ↑ Atanasov KE, Liu C, Erban A, Kopka J, Parker JE, Alcázar R. NLR Mutations Suppressing Immune Hybrid Incompatibility and Their Effects on Disease Resistance. Plant Physiol. 2018 Jul;177(3):1152-1169. PMID:29794019 doi:10.1104/pp.18.00462
- ↑ Ma S, Lapin D, Liu L, Sun Y, Song W, Zhang X, Logemann E, Yu D, Wang J, Jirschitzka J, Han Z, Schulze-Lefert P, Parker JE, Chai J. Direct pathogen-induced assembly of an NLR immune receptor complex to form a holoenzyme. Science. 2020 Dec 4;370(6521):eabe3069. PMID:33273071 doi:10.1126/science.abe3069
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