7ctn

Structure of the 328-692 fragment of FlhA (E351A/D356A)

spinqualitylabelsall models 7ctn, resolution 2.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7ctn is a 2 chain structure with sequence from Salmonella enterica subsp. enterica serovar Typhimurium str. LT2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.8Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function FLHA_SALTY Required for formation of the rod structure of the flagellar apparatus. Together with FliI and FliH, may constitute the export apparatus of flagellin. Publication Abstract from PubMed The flagellar protein export apparatus switches substrate specificity from hook-type to filament-type upon hook assembly completion, thereby initiating filament assembly at the hook tip. The C-terminal cytoplasmic domain of FlhA (FlhA(C)) serves as a docking platform for flagellar chaperones in complex with their cognate filament-type substrates. Interactions of the flexible linker of FlhA (FlhA(L)) with its nearest FlhA(C) subunit in the FlhA(C) ring is required for the substrate specificity switching. To address how FlhA(L) brings the order to flagellar assembly, we analyzed the flhA(E351A/W354A/D356A) DeltaflgM mutant and found that this triple mutation in FlhA(L) increased the secretion level of hook protein by 5-fold, thereby increasing hook length. The crystal structure of FlhA(C)(E351A/D356A) showed that FlhA(L) bound to the chaperone-binding site of its neighboring subunit. We propose that the interaction of FlhA(L) with the chaperon-binding site of FlhA(C) suppresses filament-type protein export and facilitates hook-type protein export during hook assembly. The FlhA linker mediates flagellar protein export switching during flagellar assembly.,Inoue Y, Kinoshita M, Kida M, Takekawa N, Namba K, Imada K, Minamino T Commun Biol. 2021 May 31;4(1):646. doi: 10.1038/s42003-021-02177-z. PMID:34059784 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Flagellar protein 3D structures

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