7cwd

Crystal structure of beta-galactosidase II from Bacillus circulans in complex with beta-D-galactopyranosyl disaccharide

spinqualitylabelsall models 7cwd, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7cwd is a 1 chain structure with sequence from Niallia circulans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2Å Ligands: GAL, GLC Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A6M5K904_NIACI Publication Abstract from PubMed The transgalactosylase activity of beta-galactosidase produces galacto-oligosaccharides (GOSs) with prebiotic effects similar to those of major oligosaccharides in human milk. beta-Galactosidases from Bacillus circulans ATCC 31382 are important enzymes in industrial-scale GOS production. Here, we show the high GOS yield of beta-galactosidase II from B. circulans (beta-Gal-II, Lactazyme-B), compared to other commercial enzymes. We also determine the crystal structure of the five conserved domains of beta-Gal-II in an apo-form and complexed with galactose and an acceptor sugar, showing the heterogeneous mode of transgalactosylation by the enzyme. Truncation studies of the five conserved domains reveal that all five domains are essential for enzyme catalysis, while some truncated constructs were still expressed as soluble proteins. Structural comparison of beta-Gal-II with other beta-galactosidase homologues suggests that the GOS linkage preference of the enzyme might be quite different from other enzymes. The structural information on beta-Gal-II might provide molecular insights into the transgalactosylation process of the beta-galactosidases in GOS production. High Galacto-Oligosaccharide Production and a Structural Model for Transgalactosylation of beta-Galactosidase II from Bacillus circulans.,Choi JY, Hong H, Seo H, Pan JG, Kim EJ, Maeng PJ, Yang TH, Kim KJ J Agric Food Chem. 2020 Nov 25;68(47):13806-13814. doi: 10.1021/acs.jafc.0c05871., Epub 2020 Nov 10. PMID:33169609 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Galactosidase 3D structures

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