7d5i

Structure of Mycobacterium smegmatis bd complex in the apo-form.

spinqualitylabelsall models 7d5i, resolution 2.79Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7d5i is a 2 chain structure with sequence from Mycolicibacterium smegmatis MC2 155. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.79Å Ligands: HDD, HEB Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0QXA8_MYCS2 Publication Abstract from PubMed Cytochromes bd are ubiquitous amongst prokaryotes including many human-pathogenic bacteria. Such complexes are targets for the development of antimicrobial drugs. However, an understanding of the relationship between the structure and functional mechanisms of these oxidases is incomplete. Here, we have determined the 2.8 A structure of Mycobacterium smegmatis cytochrome bd by single-particle cryo-electron microscopy. This bd oxidase consists of two subunits CydA and CydB, that adopt a pseudo two-fold symmetrical arrangement. The structural topology of its Q-loop domain, whose function is to bind the substrate, quinol, is significantly different compared to the C-terminal region reported for cytochromes bd from Geobacillus thermodenitrificans (G. th) and Escherichia coli (E. coli). In addition, we have identified two potential oxygen access channels in the structure and shown that similar tunnels also exist in G. th and E. coli cytochromes bd. This study provides insights to develop a framework for the rational design of antituberculosis compounds that block the oxygen access channels of this oxidase. Cryo-EM structure of mycobacterial cytochrome bd reveals two oxygen access channels.,Wang W, Gao Y, Tang Y, Zhou X, Lai Y, Zhou S, Zhang Y, Yang X, Liu F, Guddat LW, Wang Q, Rao Z, Gong H Nat Commun. 2021 Jul 30;12(1):4621. doi: 10.1038/s41467-021-24924-w. PMID:34330928 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Cytochrome bd oxidase

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