7dlm

Short chain dehydrogenase (SCR) crystal structure with NADPH

spinqualitylabelsall models 7dlm, resolution 1.59Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7dlm is a 2 chain structure with sequence from Candida parapsilosis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.59Å Ligands: NDP Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function B2KJ46_CANPA Publication Abstract from PubMed The evolutionary benefit accounting for widespread conservation of oligomeric structures in proteins lacking evidence of intersubunit cooperativity remains unclear. Here, crystal and cryo-EM structures, and enzymological data, demonstrate that a conserved tetramer interface maintains the active-site structure in one such class of proteins, the short-chain dehydrogenase/reductase (SDR) superfamily. Phylogenetic comparisons support a significantly longer polypeptide being required to maintain an equivalent active-site structure in the context of a single subunit. Oligomerization therefore enhances evolutionary fitness by reducing the metabolic cost of enzyme biosynthesis. The large surface area of the structure-stabilizing oligomeric interface yields a synergistic gain in fitness by increasing tolerance to activity-enhancing yet destabilizing mutations. We demonstrate that two paralogous SDR superfamily enzymes with different specificities can form mixed heterotetramers that combine their individual enzymological properties. This suggests that oligomerization can also diversify the functions generated by a given metabolic investment, enhancing the fitness advantage provided by this architectural strategy. Oligomeric interactions maintain active-site structure in a noncooperative enzyme family.,Li Y, Zhang R, Wang C, Forouhar F, Clarke OB, Vorobiev S, Singh S, Montelione GT, Szyperski T, Xu Y, Hunt JF EMBO J. 2022 Jul 8:e108368. doi: 10.15252/embj.2021108368. PMID:35801308 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Carbonyl reductase 3D structures

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