7drp

Structure of ATP-grasp ligase PsnB complexed with phosphomimetic variant of minimal precursor, Mg, and ADP

spinqualitylabelsall models 7drp, resolution 2.98Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7drp is a 6 chain structure with sequence from Plesiocystis pacifica SIR-1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.98Å Ligands: ADP, DV9, MG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A6G4D7_9DELT Publication Abstract from PubMed Graspetides, also known as omega-ester-containing peptides (OEPs), are a family of ribosomally synthesized and post-translationally modified peptides (RiPPs) bearing side chain-to-side chain macrolactone or macrolactam linkages. Here, we present the molecular details of precursor peptide recognition by the macrocyclase enzyme PsnB in the biosynthesis of plesiocin, a group 2 graspetide. Biochemical analysis revealed that, in contrast to other RiPPs, the core region of the plesiocin precursor peptide noticeably enhanced the enzyme-precursor interaction via the conserved glutamate residues. We obtained four crystal structures of symmetric or asymmetric PsnB dimers, including those with a bound core peptide and a nucleotide, and suggest that the highly conserved Arg213 at the enzyme active site specifically recognizes a ring-forming acidic residue before phosphorylation. Collectively, this study provides insights into the mechanism underlying substrate recognition in graspetide biosynthesis and lays a foundation for engineering new variants. Molecular mechanism underlying substrate recognition of the peptide macrocyclase PsnB.,Song I, Kim Y, Yu J, Go SY, Lee HG, Song WJ, Kim S Nat Chem Biol. 2021 Nov;17(11):1123-1131. doi: 10.1038/s41589-021-00855-x. Epub , 2021 Sep 2. PMID:34475564 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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