7e1r

Crystal structure of Dehydrogenase/isomerase FabX from Helicobacter pylori in complex with holo-ACP

spinqualitylabelsall models 7e1r, resolution 2.79Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7e1r is a 4 chain structure with sequence from Helicobacter pylori. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.795Å Ligands: FMN, PN7 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function B5Z7D6_HELPG Publication Abstract from PubMed Unsaturated fatty acids (UFAs) are essential for functional membrane phospholipids in most bacteria. The bifunctional dehydrogenase/isomerase FabX is an essential UFA biosynthesis enzyme in the widespread human pathogen Helicobacter pylori, a bacterium etiologically related to 95% of gastric cancers. Here, we present the crystal structures of FabX alone and in complexes with an octanoyl-acyl carrier protein (ACP) substrate or with holo-ACP. FabX belongs to the nitronate monooxygenase (NMO) flavoprotein family but contains an atypical [4Fe-4S] cluster absent in all other family members characterized to date. FabX binds ACP via its positively charged alpha7 helix that interacts with the negatively charged alpha2 and alpha3 helices of ACP. We demonstrate that the [4Fe-4S] cluster potentiates FMN oxidation during dehydrogenase catalysis, generating superoxide from an oxygen molecule that is locked in an oxyanion hole between the FMN and the active site residue His182. Both the [4Fe-4S] and FMN cofactors are essential for UFA synthesis, and the superoxide is subsequently excreted by H. pylori as a major resource of peroxide which may contribute to its pathogenic function in the corrosion of gastric mucosa. Helicobacter pylori FabX contains a [4Fe-4S] cluster essential for unsaturated fatty acid synthesis.,Zhou J, Zhang L, Zeng L, Yu L, Duan Y, Shen S, Hu J, Zhang P, Song W, Ruan X, Jiang J, Zhang Y, Zhou L, Jia J, Hang X, Tian C, Lin H, Chen HZ, Cronan JE, Bi H, Zhang L Nat Commun. 2021 Nov 26;12(1):6932. doi: 10.1038/s41467-021-27148-0. PMID:34836944 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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