7e44

Crystal structure of NudC complexed with dpCoA

spinqualitylabelsall models 7e44, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7e44 is a 4 chain structure with sequence from Escherichia coli BL21(DE3). Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2Å Ligands: COD, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function NUDC_ECOLI Publication Abstract from PubMed Various kinds of cap structures, such as m(7)G, triphosphate groups, NAD and dpCoA, protect the 5' terminus of RNA. The cap structures bond covalently to RNA and affect its stability, translation, and transport. The removal of the caps is mainly executed by Nudix hydrolase family proteins, including Dcp2, RppH and NudC. Numerous efforts have been made to elucidate the mechanism underlying the removal of m(7)G, triphosphate group, and NAD caps. In contrast, few studies related to the cleavage of the RNA dpCoA cap have been conducted. Here, we report the hydrolytic activity of Escherichia coli NudC towards dpCoA and dpCoA-capped RNA in vitro. We also determined the crystal structure of dimeric NudC in complex with dpCoA at 2.0 A resolution. Structural analysis revealed that dpCoA is recognized and hydrolysed in a manner similar to NAD. In addition, NudC may also remove other dinucleotide derivative caps of RNA, which comprise the AMP moieties. NudC homologs in Saccharomyces cerevisiae and Arabidopsis thaliana exhibited similar dpCoA decapping (deCoAping) activity. These results together indicate a conserved mechanism underpinning the hydrolysis of dpCoA-capped RNA in both prokaryotes and eukaryotes. Structural insights into dpCoA-RNA decapping by NudC.,Zhou W, Guan Z, Zhao F, Ye Y, Yang F, Yin P, Zhang D RNA Biol. 2021 Jun 18:1-10. doi: 10.1080/15476286.2021.1936837. PMID:34074215 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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