7eeb

Structure of the CatSpermasome

spinqualitylabelsall models 7eeb, resolution 2.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7eeb is a 10 chain structure with sequence from Human betaherpesvirus 5 and Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.9Å Ligands: 9Z9, BMA, NA, NAG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The cation channel of sperm (CatSper) is essential for sperm motility and fertility(1,2). CatSper comprises the pore-forming proteins CATSPER1-4 and multiple auxiliary subunits, including CATSPERbeta, gamma, delta, epsilon, zeta, and EFCAB9(1,3-9). Here we report the cryo-electron microscopy (cryo-EM) structure of the CatSper complex isolated from mouse sperm. In the extracellular view, CATSPER1-4 conform to the conventional domain-swapped voltage-gated ion channel fold(10), following a counterclockwise arrangement. The auxiliary subunits CATSPERbeta, gamma, delta and epsilon-each of which contains a single transmembrane segment and a large extracellular domain-constitute a pavilion-like structure that stabilizes the entire complex through interactions with CATSPER4, 1, 3 and 2, respectively. Our EM map reveals several previously uncharacterized components, exemplified by the organic anion transporter SLCO6C1. We name this channel-transporter ultracomplex the CatSpermasome. The assembly and organization details of the CatSpermasome presented here lay the foundation for the development of CatSpermasome-related treatments for male infertility and non-hormonal contraceptives. Structure of a mammalian sperm cation channel complex.,Lin S, Ke M, Zhang Y, Yan Z, Wu J Nature. 2021 Jul;595(7869):746-750. doi: 10.1038/s41586-021-03742-6. Epub 2021 , Jul 5. PMID:34225353 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Green Fluorescent Protein 3D structures

Proteopedia Page Contributors and Editors (what is this?)

OCA