7ef2

Crystal structure of maize SHH2 SAWADEE domain in complex with an H3K9me3 peptide

spinqualitylabelsall models 7ef2, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7ef2 is a 4 chain structure with sequence from Zea mays. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2Å Ligands: M3L, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function B7ZYP9_MAIZE Publication Abstract from PubMed RNA-directed DNA methylation (RdDM) is a plant-specific de novo DNA methylation pathway, which has extensive cross-talk with histone modifications. Here, we report that the maize RdDM regulator SAWADEE HOMEODOMAIN HOMOLOG 2 (SHH2) is an H3K9me1 reader. Our structural studies reveal that H3K9me1 recognition is achieved by recognition of the methyl group via a classic aromatic cage and hydrogen-bonding and salt-bridge interactions with the free protons of the mono-methyllysine. The di- and tri-methylation states disrupt the polar interactions, decreasing the binding affinity. Our study reveals a mono-methyllysine recognition mechanism which potentially links RdDM to H3K9me1 in maize. Recognition of H3K9me1 by maize RNA-directed DNA methylation factor SHH2.,Wang Y, Zhou X, Luo J, Lv S, Liu R, Du X, Jia B, Yuan F, Zhang H, Du J J Integr Plant Biol. 2021 Jun;63(6):1091-1096. doi: 10.1111/jipb.13103. Epub 2021, Jun 3. PMID:33913587 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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