7egv

Acetolactate Synthase from Trichoderma harzianum with inhibitor harzianic acid

spinqualitylabelsall models 7egv, resolution 2.54Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7egv is a 2 chain structure with sequence from Trichoderma harzianum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.54Å Ligands: EDO, FAD, J3L, MG, PEG, PO4, SO4, TZD Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A2N1LPC4_TRIHA Publication Abstract from PubMed Acetohydroxyacid synthase (AHAS) is the first enzyme in the branched-chain amino acid biosynthetic pathway and is a validated target for herbicide and fungicide development. Here we report harzianic acid (HA, 1) produced by the biocontrol fungus Trichoderma afroharzianum t-22 (Tht22) as a natural product inhibitor of AHAS. The biosynthetic pathway of HA was elucidated with heterologous reconstitution. Guided by a putative self-resistance enzyme in the genome, HA was biochemically demonstrated to be a selective inhibitor of fungal AHAS, including those from phytopathogenic fungi. In addition, HA can inhibit a common resistant variant of AHAS in which the active site proline is mutated. Structural analysis of AHAS complexed with HA revealed the molecular basis of competitive inhibition, which differs from all known commercial AHAS inhibitors. The alternative binding mode also rationalizes the selectivity of HA, as well as effectiveness toward resistant mutants. A proposed role of HA biosynthesis by Tht22 in the rhizosphere is discussed based on the data. Harzianic Acid from Trichoderma afroharzianum Is a Natural Product Inhibitor of Acetohydroxyacid Synthase.,Xie L, Zang X, Cheng W, Zhang Z, Zhou J, Chen M, Tang Y J Am Chem Soc. 2021 Jun 16. doi: 10.1021/jacs.1c03988. PMID:34132537 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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