7eim

Crystal Structure of the Candida Glabrata Glycogen Debranching Enzyme (W470A) in complex with maltopentaose

spinqualitylabelsall models 7eim, resolution 3.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7eim is a 2 chain structure with sequence from Candida glabrata CBS 138. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.1Å Ligands: GLC Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q6FSK0_CANGA Publication Abstract from PubMed Debranching is a critical step in the mobilization of the important energy store glycogen. In eukaryotes, including fungi and animals, the highly conserved glycogen-debranching enzyme (GDE) debranches glycogen by a glucanotransferase (GT) reaction followed by a glucosidase (GC) reaction. Previous work indicated that these reactions are catalyzed by two active sites located more than 50 A apart and provided insights into their catalytic mechanisms and substrate recognition. Here, five crystal structures of GDE in complex with oligosaccharides with 4-9 glucose residues are presented. The data suggest that the glycogen main chain plays a critical role in binding to the GT and GC active sites of GDE and that a minimum of five main-chain residues are required for optimal binding. Crystal structures of glycogen-debranching enzyme mutants in complex with oligosaccharides.,Shen M, Gong X, Xiang S Acta Crystallogr F Struct Biol Commun. 2021 Nov 1;77(Pt 11):420-426. doi:, 10.1107/S2053230X21010918. Epub 2021 Oct 29. PMID:34726181 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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