7ekd

Crystal structure of gibberellin 3-oxidase 2 (GA3ox2) in rice

spinqualitylabelsall models 7ekd, resolution 1.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7ekd is a 1 chain structure with sequence from Oryza sativa Japonica Group. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.899Å Ligands: AKG, J5X, MPD, PO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function G3OX2_ORYSJ Catalyzes the 3-beta-hydroxylation of the inactive gibberellin precursors, leading to the formation of bioactive gibberellins. In vitro, converts the precursors GA20, GA5, GA44 and GA9 to the corresponding 3-beta-hydroxylated active products GA1, GA3, GA38 and GA4, respectively. Involved in the production of bioactive GA for vegetative growth and development (PubMed:11438692). Controls the elongation of the vegetative shoot and plant height by the regulation of active gibberellin levels (Ref.7). [EMBL:BAB17075.1] Publication Abstract from PubMed Proper anther and pollen development are important for plant reproduction. The plant hormone gibberellin is important for anther development in rice, but its gametophytic functions remain largely unknown. Here, we report the functional and evolutionary analyses of rice gibberellin 3-oxidase 1 (OsGA3ox1), a gibberellin synthetic enzyme specifically expressed in the late developmental stages of anthers. Enzymatic and X-ray crystallography analyses reveal that OsGA3ox1 has a higher GA7 synthesis ratio than OsGA3ox2. In addition, we generate an osga3ox1 knockout mutant by genome editing and demonstrate the bioactive gibberellic acid synthesis by the OsGA3ox1 action during starch accumulation in pollen via invertase regulation. Furthermore, we analyze the evolution of Oryza GA3ox1s and reveal that their enzyme activity and gene expression have evolved in a way that is characteristic of the Oryza genus and contribute to their male reproduction ability. Evolutionary alterations in gene expression and enzymatic activities of gibberellin 3-oxidase 1 in Oryza.,Kawai K, Takehara S, Kashio T, Morii M, Sugihara A, Yoshimura H, Ito A, Hattori M, Toda Y, Kojima M, Takebayashi Y, Furuumi H, Nonomura KI, Mikami B, Akagi T, Sakakibara H, Kitano H, Matsuoka M, Ueguchi-Tanaka M Commun Biol. 2022 Jan 19;5(1):67. doi: 10.1038/s42003-022-03008-5. PMID:35046494 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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