7elh

In situ structure of transcriptional enzyme complex and capsid shell protein of mammalian reovirus at initiation state

spinqualitylabelsall models 7elh, resolution 3.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7elh is a 26 chain structure with sequence from Mammalian orthoreovirus 3 and Mammalian orthoreovirus 3 Dearing. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.3Å Ligands: PO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q6EDZ8_9REOV Publication Abstract from PubMed Mammalian reovirus (MRV) is the prototypical member of genus Orthoreovirus of family Reoviridae. However, lacking high-resolution structures of its RNA polymerase cofactor mu2 and infectious particle, limits understanding of molecular interactions among proteins and RNA, and their contributions to virion assembly and RNA transcription. Here, we report the 3.3 A-resolution asymmetric reconstruction of transcribing MRV and in situ atomic models of its capsid proteins, the asymmetrically attached RNA-dependent RNA polymerase (RdRp) lambda3, and RdRp-bound nucleoside triphosphatase mu2 with a unique RNA-binding domain. We reveal molecular interactions among virion proteins and genomic and messenger RNA. Polymerase complexes in three Spinoreovirinae subfamily members are organized with different pseudo-D(3d) symmetries to engage their highly diversified genomes. The above interactions and those between symmetry-mismatched receptor-binding sigma1 trimers and RNA-capping lambda2 pentamers balance competing needs of capsid assembly, external protein removal, and allosteric triggering of endogenous RNA transcription, before, during and after infection, respectively. Asymmetric reconstruction of mammalian reovirus reveals interactions among RNA, transcriptional factor micro2 and capsid proteins.,Pan M, Alvarez-Cabrera AL, Kang JS, Wang L, Fan C, Zhou ZH Nat Commun. 2021 Jul 7;12(1):4176. doi: 10.1038/s41467-021-24455-4. PMID:34234134 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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