7et0

Crystal structure of the complex formed by Wolbachia cytoplasmic incompatibility factors CinA and CinB from wPip

spinqualitylabelsall models 7et0, resolution 2.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7et0 is a 4 chain structure with sequence from Wolbachia endosymbiont of Culex quinquefasciatus Pel. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.2Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Wolbachia bacteria, inherited through the female germ line, infect a large fraction of arthropod species. Many Wolbachia strains manipulate host reproduction, most commonly through cytoplasmic incompatibility (CI). CI, a conditional male sterility, results when Wolbachia-infected male insects mate with uninfected females; viability is restored if the female is similarly infected (called "rescue"). CI is used to help control mosquito-borne viruses such as dengue and Zika, but its mechanisms remain unknown. The coexpressed CI factors CifA and CifB form stable complexes in vitro, but the timing and function of this interaction in the insect are unresolved. CifA expression in the female germ line is sufficient for rescue. We report high-resolution structures of a CI-factor complex, CinA-CinB, which utilizes a unique binding mode between the CinA rescue factor and the CinB nuclease; the structures were validated by biochemical and yeast growth analyses. Importantly, transgenic expression in Drosophila of a nonbinding CinA mutant, designed based on the CinA-CinB structure, suggests CinA expressed in females must bind CinB imported by sperm in order to rescue embryonic viability. Binding between cognate factors is conserved in an enzymatically distinct CI system, CidA-CidB, suggesting universal features in Wolbachia CI induction and rescue. Structural and mechanistic insights into the complexes formed by Wolbachia cytoplasmic incompatibility factors.,Xiao Y, Chen H, Wang H, Zhang M, Chen X, Berk JM, Zhang L, Wei Y, Li W, Cui W, Wang F, Wang Q, Cui C, Li T, Chen C, Ye S, Zhang L, Ji X, Huang J, Wang W, Wang Z, Hochstrasser M, Yang H Proc Natl Acad Sci U S A. 2021 Oct 12;118(41). pii: 2107699118. doi:, 10.1073/pnas.2107699118. PMID:34620712 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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