7etr

Crystal structure of SO_1444-SO_1445 complex from Shewanella oneidensis

spinqualitylabelsall models 7etr, resolution 3.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7etr is a 4 chain structure with sequence from Shewanella oneidensis MR-1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.804Å Ligands: MSE Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q8EGZ2_SHEON Publication Abstract from PubMed ParE(SO)-CopA(SO) is a new type II toxin-antitoxin (TA) system in prophage CP4So that plays an essential role in circular CP4So maintenance after the excision in Shewanella oneidensis. The toxin ParE(SO) severely inhibits cell growth, while CopA(SO) functions as an antitoxin to neutralize ParE(SO) toxicity through direct interactions. However, the molecular mechanism of the neutralization and autoregulation of the TA operon transcription remains elusive. In this study, we determined the crystal structure of a ParE(SO)-CopA(SO) complex that adopted an open V-shaped heterotetramer with the organization of ParE(SO)-(CopA(SO))(2)-ParE(SO). The structure showed that upon ParE(SO) binding, the intrinsically disordered C-terminal domain of CopA(SO) was induced to fold into a partially ordered conformation that bound into a positively charged and hydrophobic groove of ParE(SO). Thermodynamics analysis showed the DNA-binding affinity of CopA(SO) was remarkably higher than that of the purified TA complex, accompanied by the enthalpy change reversion from an exothermic reaction to an endothermic reaction. These results suggested ParE(SO) acts as a de-repressor of the TA operon transcription at the toxin:antitoxin level of 1:1. Site-directed mutagenesis of ParE(SO) identified His91 as the essential residue for its toxicity by cell toxicity assays. Our structure-function studies therefore elucidated the transcriptional regulation mechanism of the ParE(SO)-CopA(SO) pair, and may help to understand the regulation of CP4So maintenance in S. oneidensis. Insights into the Neutralization and DNA Binding of Toxin-Antitoxin System ParE(SO)-CopA(SO) by Structure-Function Studies.,Zhou J, Du XJ, Liu Y, Gao ZQ, Geng Z, Dong YH, Zhang H Microorganisms. 2021 Dec 3;9(12):2506. doi: 10.3390/microorganisms9122506. PMID:34946107 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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