7eyr

Fe(II)/(alpha)ketoglutarate-dependent dioxygenase SptF apo

spinqualitylabelsall models 7eyr, resolution 2.12Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7eyr is a 4 chain structure with sequence from Aspergillus sp.. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.12Å Ligands: FE2 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A6J4CX17_9EURO Publication Abstract from PubMed Non-heme iron and alpha-ketoglutarate-dependent (Fe/alphaKG) oxygenases catalyze various oxidative biotransformations. Due to their catalytic flexibility and high efficiency, Fe/alphaKG oxygenases have attracted keen attention for their application as biocatalysts. Here, we report the biochemical and structural characterizations of the unusually promiscuous and catalytically versatile Fe/alphaKG oxygenase SptF, involved in the biosynthesis of fungal meroterpenoid emervaridones. The in vitro analysis revealed that SptF catalyzes several continuous oxidation reactions, including hydroxylation, desaturation, epoxidation, and skeletal rearrangement. SptF exhibits extremely broad substrate specificity toward various meroterpenoids, and efficiently produced unique cyclopropane-ring-fused 5/3/5/5/6/6 and 5/3/6/6/6 scaffolds from terretonins. Moreover, SptF also hydroxylates steroids, including androsterone, testosterone, and progesterone, with different regiospecificities. Crystallographic and structure-based mutagenesis studies of SptF revealed the molecular basis of the enzyme reactions, and suggested that the malleability of the loop region contributes to the remarkable substrate promiscuity. SptF exhibits great potential as a promising biocatalyst for oxidation reactions. Molecular insights into the unusually promiscuous and catalytically versatile Fe(II)/alpha-ketoglutarate-dependent oxygenase SptF.,Tao H, Mori T, Chen H, Lyu S, Nonoyama A, Lee S, Abe I Nat Commun. 2022 Jan 10;13(1):95. doi: 10.1038/s41467-021-27636-3. PMID:35013177 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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