7f5n

Crystal structure of TCPTP catalytic domain

spinqualitylabelsall models 7f5n, resolution 1.93Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7f5n is a 3 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.93Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function PTN2_HUMAN Publication Abstract from PubMed The T-cell protein tyrosine phosphatase (TCPTP/PTPN2) targets a broad variety of substrates across different subcellular compartments. In spite of that, the structural basis for the regulation of TCPTP's activity remains elusive. Here, we investigated whether the activity of TCPTP is regulated by a potential allosteric site in a comparable manner to its most similar PTP family member (PTP1B/PTPN1). We determined two crystal structures of TCPTP at 1.7 and 1.9 A resolutions that include helix alpha7 at the TCPTP C-terminus. Helix alpha7 has been functionally characterized in PTP1B and was identified as its allosteric switch. However, its function is unknown in TCPTP. Here, we demonstrate that truncation or deletion of helix alpha7 reduced the catalytic efficiency of TCPTP by approximately 4-fold. Collectively, our data supports an allosteric role of helix alpha7 in regulation of TCPTP's activity, similar to its function in PTP1B, and highlights that the coordination of helix alpha7 with the core catalytic domain is essential for the efficient catalytic function of TCPTP. Crystal Structure of TCPTP Unravels an Allosteric Regulatory Role of Helix alpha7 in Phosphatase Activity.,Singh JP, Lin MJ, Hsu SF, Peti W, Lee CC, Meng TC Biochemistry. 2021 Dec 28;60(51):3856-3867. doi: 10.1021/acs.biochem.1c00519., Epub 2021 Dec 15. PMID:34910875 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Tyrosine phosphatase 3D structures

Proteopedia Page Contributors and Editors (what is this?)

OCA