7f8s

Pennisetum glaucum (Pearl millet) dehydroascorbate reductase (DHAR) with catalytic cysteine (Cy20) in sulphenic and sulfinic acid forms.

spinqualitylabelsall models 7f8s, resolution 2.63Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7f8s is a 2 chain structure with sequence from Cenchrus americanus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.63Å Ligands: CSD, CSO, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function U5XYA0_CENAM Publication Abstract from PubMed Ascorbate is an important cellular antioxidant that gets readily oxidized to dehydroascorbate (DHA). Recycling of DHA is therefore paramount in the maintenance of cellular homeostasis and preventing oxidative stress. Dehydroascorbate reductases (DHARs), in conjunction with glutathione (GSH), carry out this vital process in eukaryotes, among which plant DHARs have garnered considerable attention. A detailed kinetic analysis of plant DHARs relative to their human counterparts is, however, lacking. Chloride intracellular channels (HsCLICs) are close homologs of plant DHARs, recently demonstrated to share their enzymatic activity. This study reports the highest turnover rate for a plant DHAR from stress adapted Pennisetum glaucum (PgDHAR). In comparison, HsCLICs 1, 3, and 4 reduced DHA at a significantly lower rate. We further show that the catalytic cysteine from both homologs was susceptible to varying degrees of oxidation, validated by crystal structures and mass-spectrometry. Our findings may have broader implications on crop improvement using pearl millet DHAR vis-a-vis discovery of cancer therapeutics targeting Vitamin-C recycling capability of human CLICs. Comparative kinetic analysis of ascorbate (Vitamin-C) recycling dehydroascorbate reductases from plants and humans.,Das BK, Kumar A, Sreekumar SN, Ponraj K, Gadave K, Kumar S, Murali Achary VM, Ray P, Reddy MK, Arockiasamy A Biochem Biophys Res Commun. 2021 Dec 29;591:110-117. doi:, 10.1016/j.bbrc.2021.12.103. PMID:35007834 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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