7fiw

Crystal structure of the complex formed by Wolbachia cytoplasmic incompatibility factors CidAwMel(ST) and CidBND1-ND2 from wPip(Pel)

spinqualitylabelsall models 7fiw, resolution 2.16Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7fiw is a 2 chain structure with sequence from Wolbachia endosymbiont of Culex pipiens, Wolbachia endosymbiont of Culex quinquefasciatus Pel and Wolbachia endosymbiont of Drosophila melanogaster. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.16Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function B3CP63_WOLPP Publication Abstract from PubMed Cytoplasmic incompatibility (CI) results when Wolbachia bacteria-infected male insects mate with uninfected females, leading to embryonic lethality. "Rescue" of viability occurs if the female harbors the same Wolbachia strain. CI is caused by linked pairs of Wolbachia genes called CI factors (CifA and CifB). The co-evolution of CifA-CifB pairs may account in part for the incompatibility patterns documented in insects infected with different Wolbachia strains, but the molecular mechanisms remain elusive. Here, we use X-ray crystallography and AlphaFold to analyze the CI factors from Wolbachia strain wMel called CidA(wMel) and CidB(wMel). Substituting CidA(wMel) interface residues with those from CidA(wPip) (from strain wPip) enables the mutant protein to bind CidB(wPip) and rescue CidB(wPip)-induced yeast growth defects, supporting the importance of CifA-CifB interaction in CI rescue. Sequence divergence in CidA(wPip) and CidB(wPip) proteins affects their pairwise interactions, which may help explain the complex incompatibility patterns of mosquitoes infected with different wPip strains. Crystal Structures of Wolbachia CidA and CidB Reveal Determinants of Bacteria-induced Cytoplasmic Incompatibility and Rescue.,Wang H, Xiao Y, Chen X, Zhang M, Sun G, Wang F, Wang L, Zhang H, Zhang X, Yang X, Li W, Wei Y, Yao D, Zhang B, Li J, Cui W, Wang F, Chen C, Shen W, Su D, Bai F, Huang J, Ye S, Zhang L, Ji X, Wang W, Wang Z, Hochstrasser M, Yang H Nat Commun. 2022 Mar 25;13(1):1608. doi: 10.1038/s41467-022-29273-w. PMID:35338130 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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