7h5m

Crystal structure of endothiapepsin PN_cryo1 in complex with TL00150 at 100 K

spinqualitylabelsall models 7h5m, resolution 1.85Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7h5m is a 1 chain structure with sequence from Cryphonectria parasitica. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.853Å Ligands: ACT, DMS, U1H Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function CARP_CRYPA Publication Abstract from PubMed Advances in structural biology have relied heavily on synchrotron cryo-crystallography and cryogenic electron microscopy to elucidate biological processes and for drug discovery. However, disparities between cryogenic and room-temperature (RT) crystal structures pose challenges. Here, Cryo2RT, a high-throughput RT data-collection method from cryo-cooled crystals that leverages the cryo-crystallography workflow, is introduced. Tested on endothiapepsin crystals with four soaked fragments, thaumatin and SARS-CoV-2 3CL(pro), Cryo2RT reveals unique ligand-binding poses, offers a comparable throughput to cryo-crystallography and eases the exploration of structural dynamics at various temperatures. Cryo2RT: a high-throughput method for room-temperature macromolecular crystallography from cryo-cooled crystals.,Huang CY, Aumonier S, Olieric V, Wang M Acta Crystallogr D Struct Biol. 2024 Aug 1;80(Pt 8):620-628. doi: , 10.1107/S2059798324006697. Epub 2024 Jul 25. PMID:39052318 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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