7k5n

Ligand binding domain (tandem PAS/dCache) of Aeromonas caviae diguanylate cyclase with proline bound

spinqualitylabelsall models 7k5n, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7k5n is a 1 chain structure with sequence from Aeromonas caviae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.8Å Ligands: GOL, PRO Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A3S5WQC2_AERCA Publication Abstract from PubMed Animal microbiomes are assembled predominantly from environmental microbes, yet the mechanisms by which individual symbionts regulate their transmission into hosts remain underexplored. By tracking the experimental evolution of Aeromonas veronii in gnotobiotic zebrafish, we identify bacterial traits promoting host colonization. Multiple independently evolved isolates with increased immigration harbored mutations in a gene we named sensor of proline diguanylate cyclase enzyme (SpdE) based on structural, biochemical, and phenotypic evidence that SpdE encodes an amino-acid-sensing diguanylate cyclase. SpdE detects free proline and to a lesser extent valine and isoleucine, resulting in reduced production of intracellular c-di-GMP, a second messenger controlling bacterial motility. Indeed, SpdE binding to amino acids increased bacterial motility and host colonization. Hosts serve as sources of SpdE-detected amino acids, with levels varying based on microbial colonization status. Our work demonstrates that bacteria use chemically regulated motility, or chemokinesis, to sense host-emitted cues that trigger active immigration into hosts. Host-emitted amino acid cues regulate bacterial chemokinesis to enhance colonization.,Robinson CD, Sweeney EG, Ngo J, Ma E, Perkins A, Smith TJ, Fernandez NL, Waters CM, Remington SJ, Bohannan BJM, Guillemin K Cell Host Microbe. 2021 Aug 11;29(8):1221-1234.e8. doi:, 10.1016/j.chom.2021.06.003. Epub 2021 Jul 6. PMID:34233153 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Diguanylate cyclase

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