7k5t

Bst DNA polymerase I time-resolved structure, 25.5 hr post dATP and dCTP addition

spinqualitylabelsall models 7k5t, resolution 2.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7k5t is a 3 chain structure with sequence from Geobacillus stearothermophilus and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.3Å Ligands: DCP, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function E1C9K5_GEOSE Publication Abstract from PubMed The mechanism of DNA synthesis has been inferred from static structures, but the absence of temporal information raises longstanding questions about the order of events in one of life's most central processes. Here we follow the reaction pathway of a replicative DNA polymerase using time-resolved X-ray crystallography to elucidate the order and transition between intermediates. In contrast to the canonical model, the structural changes observed in the time-lapsed images reveal a catalytic cycle in which translocation precedes catalysis. The translocation step appears to follow a push-pull mechanism where the O-O1 loop of the finger subdomain acts as a pawl to facilitate unidirectional movement along the template with conserved tyrosine residues 714 and 719 functioning as tandem gatekeepers of DNA synthesis. The structures capture the precise order of critical events that may be a general feature of enzymatic catalysis among replicative DNA polymerases. Following replicative DNA synthesis by time-resolved X-ray crystallography.,Chim N, Meza RA, Trinh AM, Yang K, Chaput JC Nat Commun. 2021 May 11;12(1):2641. doi: 10.1038/s41467-021-22937-z. PMID:33976175 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also DNA polymerase 3D structures

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