7k7i

Density-fitted Model Structure of Antibody Variable Domains of TyTx4 in Complex with PltB pentamer of Typhoid Toxin

spinqualitylabelsall models 7k7i, resolution 3.13Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7k7i is a 15 chain structure with sequence from Mus musculus and Salmonella enterica subsp. enterica serovar Typhi. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.13Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q8Z6A3_SALTI Publication Abstract from PubMed Many bacterial pathogens secrete A((2))B(5) toxins comprising two functionally distinct yet complementary "A" and "B" subunits to benefit the pathogens during infection. The lectin-like pentameric B subunits recognize specific sets of host glycans to deliver the toxin into target host cells. Here, we offer the molecular mechanism by which neutralizing antibodies, which have the potential to bind to all glycan-receptor binding sites and thus completely inhibit toxin binding to host cells, are inhibited from exerting this action. Cryogenic electron microscopy (cryo-EM)-based analyses indicate that the skewed positioning of the toxin A subunit(s) toward one side of the toxin B pentamer inhibited neutralizing antibody binding to the laterally located epitopes, rendering some glycan-receptor binding sites that remained available for the toxin binding and endocytosis process, which is strikingly different from the counterpart antibodies recognizing the far side-located epitopes. These results highlight additional features of the toxin-antibody interactions and offer important insights into anti-toxin strategies. The structural basis of Salmonella A(2)B(5) toxin neutralization by antibodies targeting the glycan-receptor binding subunits.,Nguyen T, Richards AF, Neupane DP, Feathers JR, Yang YA, Sim JH, Byun H, Lee S, Ahn C, Van Slyke G, Fromme JC, Mantis NJ, Song J Cell Rep. 2021 Sep 7;36(10):109654. doi: 10.1016/j.celrep.2021.109654. PMID:34496256 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Antibody 3D structures

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