7l17

Crystal structure of sugar-bound melibiose permease MelB

spinqualitylabelsall models 7l17, resolution 3.05Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7l17 is a 1 chain structure with sequence from Salmonella enterica subsp. enterica serovar Typhimurium str. LT2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.05Å Ligands: 9PG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function MELB_SALTY Responsible for melibiose transport. It is capable of using hydrogen, sodium, and lithium cations as coupling cations for cotransport, depending on the particular sugar transported (symport system). Publication Abstract from PubMed Major facilitator superfamily_2 transporters are widely found from bacteria to mammals. The melibiose transporter MelB, which catalyzes melibiose symport with either Na(+), Li(+), or H(+), is a prototype of the Na(+)-coupled MFS transporters, but its sugar recognition mechanism has been a long-unsolved puzzle. Two high-resolution X-ray crystal structures of a Salmonella typhimurium MelB mutant with a bound ligand, either nitrophenyl-alpha-D-galactoside or dodecyl-beta-D-melibioside, were refined to a resolution of 3.05 or 3.15 A, respectively. In the substrate-binding site, the interaction of both galactosyl moieties on the two ligands with MelBSt are virturally same, so the sugar specificity determinant pocket can be recognized, and hence the molecular recognition mechanism for sugar binding in MelB has been deciphered. The conserved cation-binding pocket is also proposed, which directly connects to the sugar specificity pocket. These key structural findings have laid a solid foundation for our understanding of the cooperative binding and symport mechanisms in Na(+)-coupled MFS transporters, including eukaryotic transporters such as MFSD2A. X-ray crystallography reveals molecular recognition mechanism for sugar binding in a melibiose transporter MelB.,Guan L, Hariharan P Commun Biol. 2021 Aug 2;4(1):931. doi: 10.1038/s42003-021-02462-x. PMID:34341464 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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