7l3m

PEPCK MMQX structure 40ms post-mixing with oxaloacetic acid

spinqualitylabelsall models 7l3m, resolution 2.07Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7l3m is a 1 chain structure with sequence from Rattus norvegicus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.07Å Ligands: CO2, GDP, MN, NA, PEP Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function PCKGC_RAT Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Publication Abstract from PubMed Time-resolved crystallography of biomolecules in action has advanced rapidly as methods for serial crystallography have improved, but the large number of crystals and the complex experimental infrastructure that are required remain serious obstacles to its widespread application. Here, millisecond mix-and-quench crystallography (MMQX) has been developed, which yields millisecond time-resolved data using far fewer crystals and routine remote synchrotron data collection. To demonstrate the capabilities of MMQX, the conversion of oxaloacetic acid to phosphoenolpyruvate by phosphoenolpyruvate carboxy-kinase (PEPCK) is observed with a time resolution of 40 ms. By lowering the entry barrier to time-resolved crystallography, MMQX should enable a broad expansion in structural studies of protein dynamics. Millisecond mix-and-quench crystallography (MMQX) enables time-resolved studies of PEPCK with remote data collection.,Clinger JA, Moreau DW, McLeod MJ, Holyoak T, Thorne RE IUCrJ. 2021 Aug 4;8(Pt 5):784-792. doi: 10.1107/S2052252521007053. eCollection, 2021 Sep 1. PMID:34584739 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Phosphoenolpyruvate carboxykinase 3D structures

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