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DGT-28 EPSPS
Structural highlights
FunctionB5HND8_9ACTN Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.[HAMAP-Rule:MF_00210] Publication Abstract from PubMedNatural and modified versions of the 5-enolpyruvylshikimate-3-phosphate synthase (epsps) gene have been used to confer tolerance to the broad-spectrum herbicide glyphosate in a variety of commercial crops. The most widely utilized trait was obtained from the Agrobacterium tumefaciens strain CP4 and has been commercialized in several glyphosate-tolerant crops. The EPSPS gene products are enzymes that have been divided into three classes based on sequence similarity, sensitivity to glyphosate, and steady-state catalytic parameters. Herein, we describe the informatics-guided identification and biochemical and structural characterization of a novel EPSPS from Streptomyces sviceus (DGT-28 EPSPS). The data suggest DGT-28 EPSPS and other closely related homologues exemplify a distinct new class (Class IV) of EPSPS enzymes that display intrinsic tolerance to high concentrations of glyphosate (Ki >/= 5000 muM). We further demonstrate that dgt-28 epsps, when transformed into stable plants, provides robust (>/=4x field rates) vegetative/reproductive herbicide tolerance and has utility in weed-control systems comparable to that of commercialized events. Characterization of a Glyphosate-Tolerant Enzyme from Streptomyces svecius: A Distinct Class of 5-Enolpyruvylshikimate-3-phosphate Synthases.,Griffin SL, Chekan JR, Lira JM, Robinson AE, Yerkes CN, Siehl DL, Wright TR, Nair SK, Cicchillo RM J Agric Food Chem. 2021 Apr 7. doi: 10.1021/acs.jafc.1c00439. PMID:33826316[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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