7m2w

Engineered disulfide cross-linked closed conformation of the Yeast gamma-TuRC(SS)

spinqualitylabelsall models 7m2w, resolution 3.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7m2w is a 12 chain structure with sequence from Saccharomyces cerevisiae S288C. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3Å Ligands: GTP Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function TBG_YEAST Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome, suggesting that it is involved in the minus-end nucleation of microtubule assembly. TUB4 is an important spindle pole body component that organizes both cytoplasmic and nuclear microtubule arrays. Publication Abstract from PubMed Microtubule (MT) nucleation is regulated by the gamma-tubulin ring complex (gammaTuRC), conserved from yeast to humans. In Saccharomyces cerevisiae, gammaTuRC is composed of seven identical gamma-tubulin small complex (gammaTuSC) sub-assemblies, which associate helically to template MT growth. gammaTuRC assembly provides a key point of regulation for the MT cytoskeleton. Here, we combine crosslinking mass spectrometry, X-ray crystallography, and cryo-EM structures of both monomeric and dimeric gammaTuSCs, and open and closed helical gammaTuRC assemblies in complex with Spc110p to elucidate the mechanisms of gammaTuRC assembly. gammaTuRC assembly is substantially aided by the evolutionarily conserved CM1 motif in Spc110p spanning a pair of adjacent gammaTuSCs. By providing the highest resolution and most complete views of any gammaTuSC assembly, our structures allow phosphorylation sites to be mapped, surprisingly suggesting that they are mostly inhibitory. A comparison of our structures with the CM1 binding site in the human gammaTuRC structure at the interface between GCP2 and GCP6 allows for the interpretation of significant structural changes arising from CM1 helix binding to metazoan gammaTuRC. CM1-driven assembly and activation of yeast gamma-tubulin small complex underlies microtubule nucleation.,Brilot AF, Lyon AS, Zelter A, Viswanath S, Maxwell A, MacCoss MJ, Muller EG, Sali A, Davis TN, Agard DA Elife. 2021 May 5;10. pii: 65168. doi: 10.7554/eLife.65168. PMID:33949948 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Tubulin 3D Structures

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