7n2j

MicroED structure of a mutant mammalian prion segment phased by ARCIMBOLDO-BORGES

spinqualitylabelsall models 7n2j, resolution 1.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7n2j is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron crystallography, Resolution 1.5Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Electron diffraction (MicroED/3DED) can render the three-dimensional atomic structures of molecules from previously unamenable samples. The approach has been particularly transformative for peptidic structures, where MicroED has revealed novel structures of naturally occurring peptides, synthetic protein fragments, and peptide-based natural products. Despite its transformative potential, MicroED is beholden to the crystallographic phase problem, which challenges its de novo determination of structures. ARCIMBOLDO, an automated, fragment-based approach to structure determination, eliminates the need for atomic resolution, instead enforcing stereochemical constraints through libraries of small model fragments, and discerning congruent motifs in solution space to ensure validation. This approach expands the reach of MicroED to presently inaccessible peptide structures including fragments of human amyloids, and yeast and mammalian prions. For electron diffraction, fragment-based phasing portends a more general phasing solution with limited model bias for a wider set of chemical structures. Fragment-Based Ab Initio Phasing of Peptidic Nanocrystals by MicroED.,Richards LS, Flores MD, Millan C, Glynn C, Zee CT, Sawaya MR, Gallagher-Jones M, Borges RJ, Uson I, Rodriguez JA ACS Bio Med Chem Au. 2023 Feb 23;3(2):201-210. doi: , 10.1021/acsbiomedchemau.2c00082. eCollection 2023 Apr 19. PMID:37096030 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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