7n6h

The crystal structure of the GH30 subfamily 10 enzyme, AcXbh30A from Acetivibrio clariflavus

spinqualitylabelsall models 7n6h, resolution 1.28Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7n6h is a 2 chain structure with sequence from Acetivibrio clariflavus DSM 19732. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.28Å Ligands: ACT, CL Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function G8LU16_ACECE Publication Abstract from PubMed Xylobiose is a prebiotic sugar that has applications in functional foods. This report describes the first X-ray crystallographic structure models of apo and xylobiose-bound forms of a xylobiohydrolase (XBH) from Acetivibrio clariflavus. This xylan-active enzyme, a member of the recently described glycoside hydrolase family 30 (GH30), subfamily 10, phylogenetic clade has been shown to strictly release xylobiose as its primary hydrolysis product. Inspection of the apo structure reveals a glycone region X2 -binding slot. When X2 binds, the non-reducing xylose in the -2 subsite is highly coordinated with numerous hydrogen bond contacts while contacts in the -1 subsite mostly reflect interactions typical for GH30 and enzymes in clan A of the carbohydrate-active enzymes database (CAZy). This structure provides an explanation for the high functional specificity of this new bacterial GH30 XBH subfamily. The first crystal structure of a xylobiose-bound xylobiohydrolase with high functional specificity from the bacterial glycoside hydrolase family 30, subfamily 10.,St John FJ, Crooks C, Kim Y, Tan K, Joachimiak A FEBS Lett. 2022 Jul 25. doi: 10.1002/1873-3468.14454. PMID:35876256 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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