7nei
Novel Polyester Hydrolase Leipzig 7 (PHL-7)
Structural highlights
Publication Abstract from PubMedOur planet is flooded with plastics and the need for sustainable recycling strategies of polymers has become increasingly urgent. Enzyme-based hydrolysis of post-consumer plastic is an emerging strategy for closed-loop recycling of polyethylene terephthalate (PET). The polyester hydrolase PHL7 isolated from a compost metagenome completely hydrolyzed amorphous PET films, releasing 91 mg of terephthalic acid per hour and mg of enzyme. Degradation rates of the PET film of 6.8 microm h -1 were monitored by vertical scanning interferometry. Structural analysis indicated the importance of leucine at position 210 for the extraordinarily high PET-hydrolyzing activity of PHL7. Within 24 h, 0.6 mg enzyme g PET -1 completely degraded post-consumer thermoform PET packaging in an aqueous buffer at 70 degrees C without any energy-intensive pretreatments. Terephthalic acid recovered from the enzymatic hydrolysate was used to synthesize virgin PET, demonstrating the potential of polyester hydrolases as catalysts in sustainable PET recycling processes with a low carbon footprint. Low Carbon Footprint Recycling of Post-Consumer PET Plastic with a Metagenomic Polyester Hydrolase.,Sonnendecker C, Oeser J, Richter PK, Hille P, Zhao Z, Fischer C, Lippold H, Blazquez-Sanchez P, Engelberger F, Ramirez-Sarmiento CA, Oeser T, Lihanova Y, Frank R, Jahnke HG, Billig S, Abel B, Strater N, Matysik J, Zimmermann W ChemSusChem. 2021 Jun 15. doi: 10.1002/cssc.202101062. PMID:34129279[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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