7nt5

CryoEM structure of the Nipah virus nucleocapsid single helical turn assembly

spinqualitylabelsall models 7nt5, resolution 3.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7nt5 is a 14 chain structure with sequence from Escherichia coli BL21(DE3) and Henipavirus nipahense. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.5Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function NCAP_NIPAV Encapsidates the genome protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC) and serves as template for transcription and replication (By similarity). Publication Abstract from PubMed Nipah and its close relative Hendra are highly pathogenic zoonotic viruses, storing their ssRNA genome in a helical nucleocapsid assembly formed by the N protein, a major viral immunogen. Here, we report the first cryoEM structure for a Henipavirus RNA-bound nucleocapsid assembly, at 3.5 A resolution. The helical assembly is stabilised by previously undefined N- and C-terminal segments, contributing to subunit-subunit interactions. RNA is wrapped around the nucleocapsid protein assembly with a periodicity of six nucleotides per protomer, in the "3-bases-in, 3-bases-out" conformation, with protein plasticity enabling non-sequence specific interactions. The structure reveals commonalities in RNA binding pockets and in the conformation of bound RNA, not only with members of the Paramyxoviridae family, but also with the evolutionarily distant Filoviridae Ebola virus. Significant structural differences with other Paramyxoviridae members are also observed, particularly in the position and length of the exposed alpha-helix, residues 123-139, which may serve as a valuable epitope for surveillance and diagnostics. CryoEM structure of the Nipah virus nucleocapsid assembly.,Ker DS, Jenkins HT, Greive SJ, Antson AA PLoS Pathog. 2021 Jul 16;17(7):e1009740. doi: 10.1371/journal.ppat.1009740. , eCollection 2021 Jul. PMID:34270629 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Nucleoprotein 3D structures

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