7o5e

The structure of an i-motif/duplex junction at neutral pH

spinqualitylabelsall models 7o5e Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Solution NMR Ligands: DNR Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed We report here the three-dimensional structure of an i-motif/duplex junction, determined by NMR methods at neutral pH. By including a minor groove tetrad at one side of the C:C(+) stack of a monomeric i-motif, and a stem/loop hairpin at the other side, we have designed stable DNA constructs in which i-DNA and B-DNA regions coexist in a wide range of experimental conditions. This study demonstrates that i- and B-DNA are structurally compatible, giving rise to a distinctive fold with peculiar groove shapes. The effect of different residues at the i-motif/duplex interface has been explored. We also show that these constructs can be adapted to sequences of biological relevance, like that found in the promoter region of the KRAS oncogene. Structure of i-Motif/Duplex Junctions at Neutral pH.,Serrano-Chacon I, Mir B, Escaja N, Gonzalez C J Am Chem Soc. 2021 Aug 25;143(33):12919-12923. doi: 10.1021/jacs.1c04679. Epub, 2021 Aug 9. PMID:34370473 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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