7p26

Sulfated host glycan recognition by carbohydrate sulfatases of the human gut microbiota (BT4631_S1_15)

spinqualitylabelsall models 7p26, resolution 1.35Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7p26 is a 1 chain structure with sequence from Bacteroides thetaiotaomicron VPI-5482. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.35Å Ligands: 15P, CA, NA, P4K Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q89YV0_BACTN Publication Abstract from PubMed Sulfated glycans are ubiquitous nutrient sources for microbial communities that have coevolved with eukaryotic hosts. Bacteria metabolize sulfated glycans by deploying carbohydrate sulfatases that remove sulfate esters. Despite the biological importance of sulfatases, the mechanisms underlying their ability to recognize their glycan substrate remain poorly understood. Here, we use structural biology to determine how sulfatases from the human gut microbiota recognize sulfated glycans. We reveal seven new carbohydrate sulfatase structures spanning four S1 sulfatase subfamilies. Structures of S1_16 and S1_46 represent novel structures of these subfamilies. Structures of S1_11 and S1_15 demonstrate how non-conserved regions of the protein drive specificity toward related but distinct glycan targets. Collectively, these data reveal that carbohydrate sulfatases are highly selective for the glycan component of their substrate. These data provide new approaches for probing sulfated glycan metabolism while revealing the roles carbohydrate sulfatases play in host glycan catabolism. Sulfated glycan recognition by carbohydrate sulfatases of the human gut microbiota.,Luis AS, Basle A, Byrne DP, Wright GSA, London JA, Jin C, Karlsson NG, Hansson GC, Eyers PA, Czjzek M, Barbeyron T, Yates EA, Martens EC, Cartmell A Nat Chem Biol. 2022 Aug;18(8):841-849. doi: 10.1038/s41589-022-01039-x. Epub 2022, Jun 16. PMID:35710619 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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