7pm8

Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er, class 3)

spinqualitylabelsall models 7pm8, resolution 3.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7pm8 is a 4 chain structure with sequence from Amanita phalloides, Gallus gallus, Homo sapiens and Oryctolagus cuniculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.5Å Ligands: ADP, DTH, EEP, HIC, HYP, MG, PRD_002366 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function MYO5A_CHICK Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation (By similarity). Publication Abstract from PubMed The molecular motor myosin undergoes a series of major structural transitions during its force-producing motor cycle. The underlying mechanism and its coupling to ATP hydrolysis and actin binding is only partially understood, mostly due to sparse structural data on actin-bound states of myosin. Here, we report 26 high-resolution cryo-EM structures of the actomyosin-V complex in the strong-ADP, rigor, and a previously unseen post-rigor transition state that binds the ATP analog AppNHp. The structures reveal a high flexibility of myosin in each state and provide valuable insights into the structural transitions of myosin-V upon ADP release and binding of AppNHp, as well as the actomyosin interface. In addition, they show how myosin is able to specifically alter the structure of F-actin. High-resolution structures of the actomyosin-V complex in three nucleotide states provide insights into the force generation mechanism.,Pospich S, Sweeney HL, Houdusse A, Raunser S Elife. 2021 Nov 23;10. pii: 73724. doi: 10.7554/eLife.73724. PMID:34812732 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Actin 3D structures Myosin 3D Structures

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